CRO service
CRO service based on REMEDI’s know-how
- Cytotoxicity
- Whitening
- Anti-aging
- Anti-oxidant
- Anti-inflammation
- In vitro penetration & Franz cell tests
Process
※ Not only raw materials but also finished cosmetics can be tested.
Experiments
| In vitro tests | Period (weeks) | Methods | Cell lines |
|---|---|---|---|
| Cytotoxicity | 3 | CCK8 assays | Requested cell lines |
| Whitening | 4 | Absorbance (α-MSH), qPCR, WB | B16F10 |
| Anti-oxidant | 3 | CCK8 assays (H2O2) | HaCaT, HDF |
| Anti-inflammation | 3 | ELISA, qPCR, WB | RAW264.7, HaCaT |
| Cell penetration | 4 | Flow cytometry, Franz cells | Requested cell lines |
| Wound healing | 4 | Scratch assay | HaCaT |
| Pro-collagen assay | 4 | ELISA, qPCR, WB | HS68 |
| Elastase inhibition assay | 3 | Absorbance measurement | - |
In vitro efficacy tests
Conducting experiments based on our well-established in vitro systems.
-
Cytotoxicity
- Cell line
- Requested cell lines
- Time
- 24-48 h
- Using plate
- 96-well
- Positive control
- Doxorubicin
-
Celll permeability
- Cell line
- Requested cell lines or Franz cells
- Time
- 2-24 h
- Using plate
- 24-well
- Positive control
- TAT-FITC, TAT-EGFP
-
Whitening
- Cell line
- B16F10
- Time
- 48-72 h
- Using plate
- 24-well
- Positive control
- Arbutin, Kojic acid
-
Wound healing assay
- Cell line
- HACAT
- Time
- ~24 h
- Using plate
- 24-well
- Positive control
- EGF or madecassoside
-
Antioxidant effect
- Cell line
- HACAT
- Time
- ~24 h
- Using plate
- 24-well
- Positive control
- EGF or madecassoside
-
Pro-collagen assay
This assay can be done by ELISA, qPCR.
- Cell line
- HS68
- Time
- 48~72 h
- Using plate
- 24-well
- Positive control
- Ascorbic acid
-
Anti-inflammatory effect
This assay can be done by qPCR, WB.
- Cell line
- RAW264.7, THP-PMA
- Time
- 48-72 h
- Using plate
- 24-well
- Positive control
- LPS
-
In vitro assay
- Cell line
- No cells needed
- Time
- ~3 h
- Using plate
- 96-well
- Positive control
- Ascorbic acid