CRO service

CRO service

CRO service based on REMEDI’s know-how

  • Cytotoxicity
  • Whitening
  • Anti-aging
  • Anti-oxidant
  • Anti-inflammation
  • In vitro penetration & Franz cell tests

Process

ProcessProcess

※ Not only raw materials but also finished cosmetics can be tested.

Experiments

In vitro tests Period (weeks) Methods Cell lines
Cytotoxicity 3 CCK8 assays Requested cell lines
Whitening 4 Absorbance (α-MSH), qPCR, WB B16F10
Anti-oxidant 3 CCK8 assays (H2O2) HaCaT, HDF
Anti-inflammation 3 ELISA, qPCR, WB RAW264.7, HaCaT
Cell penetration 4 Flow cytometry, Franz cells Requested cell lines
Wound healing 4 Scratch assay HaCaT
Pro-collagen assay 4 ELISA, qPCR, WB HS68
Elastase inhibition assay 3 Absorbance measurement -

In vitro efficacy tests

Conducting experiments based on our well-established in vitro systems.

  • Cytotoxicity

    Cell line
    Requested cell lines
    Time
    24-48 h
    Using plate
    96-well
    Positive control
    Doxorubicin
  • Celll permeability

    Cell line
    Requested cell lines or Franz cells
    Time
    2-24 h
    Using plate
    24-well
    Positive control
    TAT-FITC, TAT-EGFP
  • Whitening

    Cell line
    B16F10
    Time
    48-72 h
    Using plate
    24-well
    Positive control
    Arbutin, Kojic acid
  • Wound healing assay

    Cell line
    HACAT
    Time
    ~24 h
    Using plate
    24-well
    Positive control
    EGF or madecassoside
  • Antioxidant effect

    Cell line
    HACAT
    Time
    ~24 h
    Using plate
    24-well
    Positive control
    EGF or madecassoside
  • Pro-collagen assay

    This assay can be done by ELISA, qPCR.

    Cell line
    HS68
    Time
    48~72 h
    Using plate
    24-well
    Positive control
    Ascorbic acid
  • Anti-inflammatory effect

    This assay can be done by qPCR, WB.

    Cell line
    RAW264.7, THP-PMA
    Time
    48-72 h
    Using plate
    24-well
    Positive control
    LPS
  • In vitro assay

    Cell line
    No cells needed
    Time
    ~3 h
    Using plate
    96-well
    Positive control
    Ascorbic acid